Equine Genotypes™ Panel 1.1

Microsatellite assay for horse parentage testing and identification

Equine Genotypes™ Panel 1.1 is a reagent kit for horse parentage testing and identification using microsatellite (STR) DNA loci. The kit allows co-amplification of the microsatellites in a single multiplex PCR reaction.

The Equine Genotypes™ Panel 1.1 encompasses the following 17 loci: VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425. These include the nine loci recommended by the Equine Genetics and Thoroughbred Parentage Testing Standardization Committee of the International Society for Animal Genetics (ISAG) and eight additional loci commonly used for horse parentage testing and identification.

Reagents and protocols of the Equine Genotypes™ Panel 1.1. kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. The kit employs Phusion® Hot Start DNA Polymerase. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. This is due to the proofreading activity of the Phusion Hot Start Polymerase.

The Equine Genotypes™ Panel 1.1 allows co-amplification of the 17 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (Figure 1).

The Equine Genotypes™ Panel 1.1. provides all the necessary reagents for amplification of the 17 microsatellite loci. In addition, the kit includes equine control DNA for verifying acceptable PCR and electrophoresis conditions.

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Figure 1. Representative results for the Equine Genotypes™ Panel 1.1 using control DNA and template DNA extracted from hair.

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