Bovine Genotypes™ Panels 1.2, 2.2 and 3.1

We offer three reagent kits for cattle parentage testing and identification using microsatellite (STR) loci. The kits allow co-amplification of the microsatellites in a single multiplex PCR reaction. The reagents and protocols of the kits have been optimized to deliver similar peak sizes (amplification yields) for each loci. The kits employ Phusion Hot Start DNA Polymerase. Allele callings obtained with the kits represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. This is due to the proofreading activity of the Phusion DNA Polymerase.

Bovine Genotypes™ Panel 1.2 (12 STR loci)

Bovine Genotypes™ Panel 1.2 encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in bovine parentage testing and identification, including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225 and BM1824. The primer sequences employed by the kit are identical to those recommended by the Cattle Molecular Markers and Parentage Testing standing committee of ISAG and they have been validated in extensive laboratory comparison tests.

The Bovine Genotypes™ Panel 1.2. allows co-amplification of the 12 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (Figure 1).

The Bovine Genotypes™ Panel 1.2 provides all the necessary reagents for amplification of the 12 microsatellite loci. In addition, the kit includes bovine control DNA for verifying acceptable PCR and electrophoresis conditions.

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Figure 1. Representative results for the Bovine Genotypes™ Panel 1.2 using control DNA and template DNA extracted from hair.

Bovine Genotypes™ Panel 2.2 (6 STR loci)

Bovine Genotypes™ Panel 2.2 encompasses the following six STR loci: SPS113, RM067, CSRM60, MGTG4B, CSSM66 and ILSTS006. These are among the list of loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.

The Bovine Genotypes™Panel 2.2 is intended to be used as a complement to the Bovine Genotypes™ Panel 1.2 when more STR loci are required for resolving a parentage case. The microsatellite alleles of the Bovine Genotypes™ Panel 2.2 kit can be separated and detected in the same single electrophoresis injection with the microsatellite alleles of the Bovine Genotypes™ Panel 1.2 kit (Figure 2).

The Bovine Genotypes™ Panel 2.2 allows co-amplification of the six microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (Figure 2).

The Bovine Genotypes™ Panel 2.2 provides all the necessary reagents for amplification of the six microsatellite loci. In addition, the kit includes bovine control DNA for verifying acceptable PCR and electrophoresis conditions.

This product has been developed in collaboration with Maxxam Analytics Inc.

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Figure 2. Representative results for the Bovine Genotypes™ Panel 2.2 when analyzed in the same electrophoresis injection with Panel 1.2 microsatellites using control DNA and template DNA extracted from hair.

Bovine Genotypes™ Panel 3.1 (18 STR loci)

Bovine Genotypes™ Panel 3.1 encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in parentage testing and identification, including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824 and BM1818. In addition, the kit includes the following six microsatellites which are among the list of loci recommended by the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals: SPS113, RM067, CSRM60, MGTG4B, CSSM66 and ILSTS006. Bovine Genotypes™ Panel 3.1 is a combination of Panels 1.2 and 2.2 introduced above thus enabling the analysis of both panels in a single reaction.

The Bovine Genotypes™ Panel 3.1 allows co-amplification of the 18 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (Figure 3).

The Bovine Genotypes™ Panel 3.1 provides all the necessary reagents for amplification of the 18 microsatellite loci. In addition, the kit includes bovine control DNA for verifying acceptable PCR and electrophoresis conditions.

This product has been developed in collaboration with Maxxam Analytics Inc.

Click pictures for a full-sized image
Figure 3. Representative results for the Bovine Genotypes™ Panel 3.1 using control DNA and template DNA extracted from hair.

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