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PathoProof™ Mastitis PCR Assay
Bacteria detected by the test
PathoProof™ Mastitis Complete-12 Kit identifies the following bacterial species and groups:
- Staphylococcus aureus
- Enterococcus sp. (including E. faecalis and E. faecium)
- Corynebacterium bovis
- Staphylococcal β-lactamase gene (the gene responsible for penicillin resistance in staphylococci)
- Escherichia coli
- Streptococcus dysgalactiae
- Staphylococcus sp. (including Staphylococcus aureus and all major coagulase-negative staphylococci)
- Streptococcus agalactiae
- Streptococcus uberis
- Klebsiella sp. (including K. oxytoca and K. pneumoniae)
- Serratia marcescens
- Arcanobacterium pyogenes and/or Peptoniphilus indolicus
PathoProof™ Mastitis Major-3 Kit identifies the following bacterial species:
- Staphylococcus aureus
- Streptococcus agalactiae
- Mycoplasma bovis
Large-scale prevalence studies indicate that >95 % of all clinical and subclinical mastitis cases are caused by the species identified by the PathoProof Mastitis PCR Assay.
Makovec, J.A.and Ruegg P.L. (2003) Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8,905 samples (1994–2001). J. Am. Vet. Med. Assoc. 222: 1582–1589.
Tenhagen B.A. et al. (2006) Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J. Dairy Sci. 89: 2542–2551.
Koivula M. et al. (2007) Distribution of bacteria and seasonal and regional effects in a new database for mastitis pathogens in Finland. Acta Agriculturae Scand. Section A 57: 89–96.
