PathoProof™ Mastitis PCR Assay

From sample to results in four hours

The PathoProof Mastitis PCR Assay is performed directly from milk samples. No bacterial culturing steps are required. The results are obtained in just four hours, with only two hours of hands-on time. As conventional bacterial culturing routinely take up to 48 hours, the PathoProof Mastitis PCR Assay gives significant time savings in mastitis testing.

Workflow

1.	DNA extraction DNA of the bacteria present in bovine milk is isolated and purified. In this step, bacterial DNA is released by breaking the cells and purified from all other components in milk such as lipids, proteins and bacterial cell debris. The DNA extraction protocol of the PathoProof Mastitis PCR Assay has been developed for use with even the most difficult samples from cows having mastitis.	2 h
2.	DNA amplification Bacterial DNA is amplified using real-time PCR. In this step, species-specific regions of DNA are simultaneously amplified and detected by a real-time PCR instrument. Read more...	1.5 h
3.	Analysis The data obtained from the real-time PCR step are interpreted, reported and stored with a special software. Read more...	30 min
	Total time required	4 h

Back to PathoProof Mastitis PCR Assay main page Bacteria detected by the test FAQ DNA amplification with real-time PCR technology Results interpretation made easy Collaboration with Qiagen and Agilent Technologies
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PathoProof™ Mastitis PCR Assay brochure Instruction manuals Articles Reference customers

Instrument-specific template files Norden Lab Mastitis Studio General Edition

System components and ordering information Starter Pack

From sample to results in four hours

Workflow

2 h

1.5 h

30 min